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Oxford University Hospitals NHS Foundation Trust

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MDS / MPN (M224)

Molecular investigations

Detection of the BCR-ABL fusion gene - screen for the presence of BCR-ABL fusion transcripts using multiplex PCR.

JAK2 V617F - targeted analysis by allele specific PCR and pyrosequencing. Appropriate samples are forwarded to the Wessex Genetics Laboratory for analysis of the MPN panel.

Myeloid panel (multi-target NGS panel (small variant)).

Karyotyping

As outlined in the Test Directory.

FISH

As outlined in the Test Directory other than M224.9, M224.27, M224.31 & M224.32.

Please contact us to discuss forwarding the sample elsewhere for FISH investigations.

Last reviewed:02 August 2021