Clostridium difficile (C.diff)

Some strains also make the toxins TcdA and TcdB, which have been identified as major C.difficile virulence factors and are the primary markers for diagnosis of C.difficile disease.

Sample type

Faeces

Specimen requirements

Non-formed stools from symptomatic patients (three or more episodes of type 5-7 stools within 24 hours, not attributable to any other cause or therapy)

Formed stools, and stools from patients that have tested positive for C.difficile in the previous 21 days, will not be tested (definition of formed stool: does not conform to the shape of the sample pot)

Faeces should be sent in a sterilin 30ml universal with quick-start cap and spoon

Minimum volume

1-2g

Frequency of test

Daily

Turnaround time

C.diff GDH - one day

C.diff Toxin A&B - one day

What to request on EPR

Clostridium difficile toxin, faeces